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We evaluate the instantaneous radiative forcing (IRF) of tropospheric ozone predicted by four state‐of‐the‐art global chemistry climate models (AM2‐Chem, CAM‐Chem, ECHAM5‐MOZ, and GISS‐PUCCINI) against ozone distribution observed from the NASA Tropospheric Emission Spectrometer (TES) during August 2006. The IRF is computed through the application of an observationally constrained instantaneous radiative forcing kernels (IRFK) to the difference between TES and model‐predicted ozone. The IRFK represent the sensitivity of outgoing longwave radiation to the vertical and spatial distribution of ozone under all‐sky condition. Through this technique, we find total tropospheric IRF biases from −0.4 to + 0.7 W/m2 over large regions within the tropics and midlatitudes, due to ozone differences over the region in the lower and middle troposphere, enhanced by persistent bias in the upper troposphere‐lower stratospheric region. The zonal mean biases also range from −30 to +50 mW/m2 for the models. However, the ensemble mean total tropospheric IRF bias is less than 0.2 W/m2 within the entire troposphere.