TROPOspheric Monitoring Instrument (TROPOMI), on-board the Sentinel-5 Precurser satellite, is a nadir-viewing spectrometer measuring reflected sunlight in the ultraviolet, visible, near-infrared, and shortwave infrared. From these spectra several important air quality and climate-related atmospheric constituents are retrieved, including nitrogen dioxide (NO2 ) at unprecedented spatial resolution from a satellite platform. We present the first retrievals of TROPOMI NO2 over the Canadian Oil Sands, contrasting them with observations from the Ozone Monitoring Instrument satellite instrument, and demonstrate TROPOMI's ability to resolve individual plumes and highlight its potential for deriving emissions from individual mining facilities. Further, the first TROPOMI NO2 validation is presented, consisting of aircraft and surface in situ NO2 observations, and ground-based remote-sensing measurements between March and May 2018. Our comparisons show that the TROPOMI NO2 vertical column densities are highly correlated with the aircraft and surface in situ NO2 observations, and the ground-based remote-sensing measurements with a low bias (15–30 %); this bias can be reduced by improved air mass factors. Plain Language Summary Nitrogen dioxide (NO2 ) is a pollutant that is linked to respiratory health issues and has negative environmental impacts such as soil and water acidification. Near the surface the most significant sources of NO2 are fossil fuel combustion and biomass burning. With a recently launched satellite instrument (TROPOspheric Monitoring Instrument [TROPOMI]), NO2 can be measured with an unprecedented combination of accuracy, spatial coverage, and resolution. This work presents the first TROPOMI NO2 measurements near the Canadian Oil Sands and shows that these measurements have an outstanding ability to detect NO2 on a very high horizontal resolution that is unprecedented for satellite NO2 observations. Further, these satellite measurements are in excellent agreement with aircraft and ground-based measurements.