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Nitrous oxide (N2 O) is the fourth most important greenhouse gas in the atmosphere and is considered the most important current source gas emission for global stratospheric ozone depletion (O3 ). It has natural and anthropogenic sources, mainly as an unintended by-product of food production activities. This work examines the identification and quantification of trends in the N2 O concentration from the middle troposphere to the middle stratosphere (MTMS) by in situ and remote sensing observations. The temporal variability of N2 O is addressed using a comprehensive dataset of in situ and remote sensing N2 O concentrations based on aircraft and balloon measurements in the MTMS from 1987 to 2018. We determine N2 O trends in the MTMS, based on observations. This consistent dataset was also used to study the N2 O seasonal cycle to investigate the relationship