[Exports_project_office] TM sampling on EXPORTS

Fri Jul 27 13:28:57 PDT 2018

Looks fine to me.  

Jason Graff
Assistant Professor - Senior Research
Oregon State University
Department of Botany & Plant Pathology
jrgraff at science.oregonstate.edu
541-737-4090

On Jul 27, 2018, at 11:40 AM, "Cetinic, Ivona (GSFC-616.0)[UNIVERSITIES SPACE RESEARCH ASSOCIATION]" <ivona.cetinic at nasa.gov> wrote:

> Hello to all,
> 
> a late sampling request. It is for plankton cellular metal measurements. I don't think it is competing with anyone. 
> 
> Also - it seems that there is no additional water needs. 
> 
> Pls let me know if there is a problem with this so I can give an ok go
> 
> TY all
> 
> Ivona
> 
> Ivona Cetinic, Ph.D.
> NASA Goddard Space Flight Center/USRA
> Ocean Ecology Laboratory
> Code 616
> Greenbelt, MD 20771, USA
> Phone: +1-301-286-1514
> 
> "You can observe a lot just by watchin'." Yogi Berra
> From: Benjamin Twining [btwining at bigelow.org]
> Sent: Thursday, July 26, 2018 11:11 PM
> To: Marchetti, Adrian
> Cc: Cetinic, Ivona (GSFC-616.0)[UNIVERSITIES SPACE RESEARCH ASSOCIATION]; Peter Morton; Craig, Susanne E. (GSFC-6160)[UNIVERSITIES SPACE RESEARCH ASSOCIATION]; David Siegel; Buck, Kristen; Jason Graff; Debbie Steinberg
> Subject: Re: TM sampling on EXPORTS
> 
> Hi all,
> 
> Thanks for considering this late request. 
> 
> I have filled out the EXPORTS sampling form here:
> 
> https://docs.google.com/document/d/1B5nbeN-M_0q35Cj2aYbwdvwVke7Sym-02wkd0Dn5TEM/edit?usp=sharing
> 
> No additional sampling is required for this. The goal is to get some samples of phytoplankton for cellular metal measurements that can be combined and compared with Adrian's transcriptomic measurements. Samples will only be taken (by Pete Morton) from depths from which Adrian is sampling.
> 
> best,
> 
> Ben
> 
> 
> On Thu, Jul 26, 2018 at 10:18 PM, Marchetti, Adrian <amarchet at email.unc.edu> wrote:
> Thanks Ivona.
> 
>   I found the link for the collaborator form although it does not appear to be active any longer.  Alternatively, Ben, at the link below are the questions on the form that Melissa Duhaime filled out as we are collecting samples for her for marine viruses.  This should provide you with the information they are requesting.  In relation to water needs, there are no significant additional water needs as Ben's samples can be taken from my groups water budget allocations.  
> 
> EXPORTS sample collection form
> 
> Thanks,
> Adrian
> 
> From: "ivona.cetinic" <ivona.cetinic at nasa.gov>
> Date: Thursday, July 26, 2018 at 11:40 AM
> To: Adrian Marchetti <amarchet at email.unc.edu>, Peter Morton <pmorton at fsu.edu>, Benjamin Twining <btwining at bigelow.org>, "Craig, Susanne E. (GSFC-6160)[UNIVERSITIES SPACE RESEARCH ASSOCIATION]" <susanne.e.craig at nasa.gov>, David Siegel <david.siegel at ucsb.edu>
> Cc: "Buck, Kristen" <kristenbuck at usf.edu>, Jason Graff <jrgraff at science.oregonstate.edu>, Debbie Steinberg <debbies at vims.edu>
> 
> Subject: Re: TM sampling on EXPORTS
> 
> Hello Adrian et all,
> 
> Usual procedure is to put the request in (deadline was like 2 months ago) so we can evaluate if the suggested sampling is not in competition with science that has been funded under exports. 
> 
> So answer is yes, i need a write up and i will take it with office. Sooner the better. 
> 
> Also, please discuss potential changes in water budget with chief scientists, i have cced them here. 
> 
> Thank you
> 
> Ivona
> 
> 
> 
> 
> 
> Sent from my Sprint Samsung Galaxy S8.
> 
> -------- Original message --------
> From: "Marchetti, Adrian" <amarchet at email.unc.edu>
> Date: 7/26/18 9:31 AM (GMT-05:00)
> To: Peter Morton <pmorton at fsu.edu>, Benjamin Twining <btwining at bigelow.org>
> Cc: "Buck, Kristen" <kristenbuck at usf.edu>, "Cetinic, Ivona (GSFC-616.0)[UNIVERSITIES SPACE RESEARCH ASSOCIATION]" <ivona.cetinic at nasa.gov>
> Subject: Re: TM sampling on EXPORTS
> 
> Pete et al.
> 
>   Attached is our sampling teams schedule.  The molecular sampling is indicated on point f.  In terms of water needs, there should be enough water from the depth we are performing the molecular analysis for your measurements. There are 6 bottles being triggered at depth 1 on the TMC 1 cast and the water needs are currently at 70L, leaving 2 L unclaimed (6 X 12 L = 72L).  Also, the 40L I requested is more than I will need so there should also be some spare there.
> 
>   I’ve included Ivona on this e-mail just to let her know about the additional sampling.  Ivona, let us know if there is anything else you need from us in relation to this added minor sampling effort.  Should we be filling out the new collaborator form?  If so, can you resend us the link?
> 
> Thanks,
> Adrian
> 
> *****************************************************
> Adrian Marchetti, Ph.D.
> Department of Marine Sciences
> University of North Carolina at Chapel Hill
> Phone: 919-843-3473
> Website: http://marchettilab.web.unc.edu/
> ******************************************************
> 
> From: Peter Morton <pmorton at fsu.edu>
> Date: Thursday, July 26, 2018 at 8:57 AM
> To: Benjamin Twining <btwining at bigelow.org>, Adrian Marchetti <amarchet at email.unc.edu>
> Cc: "Buck, Kristen" <kristenbuck at usf.edu>
> Subject: RE: TM sampling on EXPORTS
> 
> Hello all,
>  
> I have the supplies ready, and ’ll coordinate with Adrian to collect those SXRF samples from Adrian’s casts. I imagine that there will be plenty of targets in a small amount of water from this cruise. So, I propose to aim for 1 L of water volume, settle for 0.5 L, and take as much as 2 L to filter for SXRF samples.
>  
> Adrian: please let me know if you think this volume request is compatible with your requirements.
>  
> Ben: I’m happy to set aside the entire filter for you. If you’d like Berger/Total concentrations from the samples to complement what you and Adrian are studying, I can process your samples alongside the other particulate samples I’ll get from Kristen et al.
>  
> Pete
>  
> From: Benjamin Twining <btwining at bigelow.org> 
> Sent: Wednesday, July 25, 2018 5:12 PM
> To: Marchetti, Adrian <amarchet at email.unc.edu>
> Cc: Buck, Kristen <kristenbuck at usf.edu>; Peter Morton <pmorton at fsu.edu>
> Subject: Re: TM sampling on EXPORTS
>  
> Adrian,
>  
> Thanks for the reminder about Scott Giffords collections -- I'll stick with phytoplankton samples.
>  
> Ben
>  
> On Wed, Jul 25, 2018 at 5:10 PM, Marchetti, Adrian <amarchet at email.unc.edu> wrote:
> Hi All,
>  
>   Our metatranscriptomics sampling will be at depth 1 (near surface) within the mixed layer.  Pete, we can co-ordinate this during TMC 1 cast, which is when we will be collecting samples for RNA sequencing.  Ben if you interested, Scott Gifford will be collecting metagenomic samples of bacteria at depths 4 and 5 (around 10% and 1% Io) in addition to depth 1 on TMC 1.
>  
> Adrian
>  
> *****************************************************
> Adrian Marchetti, Ph.D.
> Department of Marine Sciences
> University of North Carolina at Chapel Hill
> Phone: 919-843-3473
> Website: http://marchettilab.web.unc.edu/
> ******************************************************
>  
> From: Benjamin Twining <btwining at bigelow.org>
> Date: Wednesday, July 25, 2018 at 5:01 PM
> To: Adrian Marchetti <amarchet at email.unc.edu>
> Cc: "Buck, Kristen" <kristenbuck at usf.edu>, Pete Morton <pmorton at fsu.edu>
> 
> Subject: Re: TM sampling on EXPORTS
>  
> Hi all,
>  
> Sorry to let this email lag -- where did July go?!?! 
>  
> I'm writing to clarify what was concluded:
>  
> Given everything already planned by Kristen and Pete, I'm just interested in getting SXRF samples on PC membranes (or Isopore) from depths/locations where Adrian takes transcriptomics samples.  If a few of these can be collected, that would be fantastic. If not possible, I understand, as well. These samples could help Adrian and me extend our collaboration connecting phytoplankton physiology with metal contents in the northeast Pacific.
>  
> Pete, these would be unfixed and dried and stored at ambient in petri slides. Splits from Berger/total digestions would be fine, as well.
>  
> good luck on the cruise,
>  
> Ben
>  
> On Fri, Jun 29, 2018 at 11:36 PM, Marchetti, Adrian <amarchet at email.unc.edu> wrote:
> Hi All,
>  
>   It’s great to hear of the possibility to collect samples for Ben.  Two things to keep in mind, first it would be great for him to have samples that are at the same depth we are collecting for the molecular work (which is only depth 1, or roughly 5 m?).  Second, we will need to let Ivona and others know we are collecting these samples and likely fill out the collaborator form they produced.  I’ll try and find the link for this or just e-mail Ivona directly.  Ben, Scott Gifford will also be collecting for bacterial genomics at a few other depths on the TMC cast if that is also of interest to you although all the depths we are sampling at on the TMC rosette are within the euphotic zone.
>  
> Cheers,
> Adrian
>  
> *****************************************************
> Adrian Marchetti, Ph.D.
> Department of Marine Sciences
> University of North Carolina at Chapel Hill
> Phone: 919-843-3473
> Website: http://marchettilab.web.unc.edu/
> ******************************************************
>  
> From: "Buck, Kristen" <kristenbuck at usf.edu>
> Date: Wednesday, June 27, 2018 at 1:58 PM
> To: Benjamin Twining <btwining at bigelow.org>
> Cc: Pete Morton <pmorton at fsu.edu>, Adrian Marchetti <amarchet at email.unc.edu>
> Subject: Re: TM sampling on EXPORTS
>  
> Hey Ben,
>  
> I’m not entirely sure, but I think Phoebe is getting samples from both.
>  
> Definitely talk to Pete, he just mentioned that he thinks you two could share filters. Apparently he’s only doing frustule work with the SXRF so the intracellular work you do would of course be a great complement if we can work out the logistics. That’s assuming I understood all that correctly… ;-)
>  
> Also, we are doing the same type of work on 3-8 day growout experiments with Bethany Jenkins. I expect these to have more biomass and might be a good way to get some additional filters for you and Pete, too. 
>  
> Cheers,
> Kristen
>  
> On Jun 27, 2018, at 11:15, Benjamin Twining <btwining at bigelow.org> wrote:
>  
> Thanks, Kristen. It sounds like you and Pete have things covered well. I'll talk separately with Pete about whether it is possible (and/or worthwhile) for me to try to do any additional analyses on the samples he collects.  Pete can coordinate with Travis and Salvo if anything additional is possible or needed.
>  
> Will Phoebe be doing particles from pumps, or traps, or both?
>  
> Ben
>  
> On Wed, Jun 27, 2018 at 9:35 AM, Buck, Kristen <kristenbuck at usf.edu> wrote:
> Hey Ben et al.,
>  
> Morning! Yes, we are collecting both dissolved and particulate samples on EXPORTS. My lab is doing the Berger leach on the filters, and Pete is doing total digests on them after we are done. Wherever we have the water budget and/or biomass to support replicate filters, Pete is planning to take those for Berger/totals and splits for SXRF. We don’t anticipate getting many field samples since the water budgets are super tight and the emphasis is on experiments rather than water column sampling on the Revelle. I have offered to collect dissolved and particulate samples from any of the TM experiments conducted on the cruise, and am still happy to do that. Pete is really the best person to talk to about particle analyses from those samples since I don’t want to encroach on his plans for the filters. He’s also going to
> 
> 
> 
> -- 
> Benjamin S. Twining, PhD
> Vice President for Education
> Senior Research Scientist
> Research Faculty, Colby College
> Bigelow Laboratory for Ocean Sciences
> 60 Bigelow Drive
> P.O. Box 380
> East Boothbay, ME 04544 
> Tel:  207-315-2567 ext 309
> Fax: 207-315-2329
> email: btwining at bigelow.org
> web: www.bigelow.org
> @DrSingleCell
> 
> 
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