[Exports_pi] Particle size analysis

Thu Aug 2 06:53:56 PDT 2018

Andrew,
I am sorry about this.
Most of our equipment is not going to be available until Monday am. We can
try to calibrate Monday pm if we can get it all set up, but I would not
hold my breath that we will have everything ready. For the LISST I would
suggest running 0.2um filtered seawater (Ivona will produce some) and beads
varying from 0.9um to 10 or 20um. The beads could be used after the cruise
to check that the VCC number (used for the amplitude of the PSD and
supplied by Sequoia) is consistent. Running several concentration for each
beads will help you avoid issues of bead blanks (using change in beam
attenuation to compute the concentrations of added beads between additions).
I just used a LISST-deep and must say I don't like its calibration setup
(the piece of plastic with hose clamps). It requires a bunch of cleaning
cycles once on the instrument and time to get rid of bubbles.
Best,
  Emmanuel

On Thu, Aug 2, 2018 at 2:35 AM Andrew McDonnell <amcdonnell at alaska.edu>
wrote:

> Hello Emmanuel, Susanne, et al..,
>
> I’m just coming back online and catching up with email.
>
> With regard to the APL cross calibration work, I originally thought that
> would be done before the mobilization, then on Monday, but now I see it on
> the mob calendar for Tuesday and Wednesday.  Tuesday (and maybe Wednesday)
> are the days my team will be needing to install the LISST DEEP instruments
> on the respective rosettes during the mobilization, so that makes it
> difficult to participate in the cross-calibration activities.  Sorry if I
> missed the conversation, but is there any way we can stick with the
> original plan and do it on Monday?  Otherwise, We can try to do the same
> inter calibration measurements with the LISST DEEP at a different time, or
> at the same time but different places (while they are mounted to the
> rosettes).
>
> For the LISST DEEPs, I will plan on using some of Emmanuel's bead stock
> and any available culture material to keep things consistent for the cross
> calibrations.
>
> Andrew
>
>
>
> On Jul 23, 2018, at 7:20 AM, Emmanuel Boss <emmanuel.boss at maine.edu>
> wrote:
>
> PS
> A paper on the comparison between the in-line LISST and a Coulter Counter
> (as well as the IFCB) from a recent NAAMES cruise can be found at:
> http://misclab.umeoce.maine.edu/documents/Bossetal2018.pdf
>
> Generally speaking PSDs compared well in shape but with a significant
> difference in magnitude between LISST and CC. Comparison with other optical
> devices, biogeochemical measurements, as well as IFCB-dervied PSD of
> chlorophyll containing particles suggested the LISST was consistent.
> Best,
>   Emmanuel
>
>
>
> On Mon, Jul 23, 2018 at 11:15 AM Emmanuel Boss <emmanuel.boss at maine.edu>
> wrote:
>
>> Susanne and all,
>> We will be running a LISST as part of our in-line measurements on board
>> the Ravelle.
>>
>> We are planning on running beads through the LISST and other optical
>> sizing sensors prior to embarking on the cruise as part of the optical
>> cross-calibration exercise.
>> We should have sufficient numbers of beads available if others would like
>> to use them as well.
>> We use optical measurements based on published properties of the beads to
>> constrain the beads' concentration.
>> The beads are spherical, nearly mono-dispersed, and much more refractive
>> compared to oceanic particles.
>>
>> We will also be bringing and running cultures through the different
>> instruments, primarily to compare sizing capabilities (as opposed to
>> absolute concentrations), though these will also be assessed when on board.
>> Best,
>>    Emmanuel
>>
>>
>>
>> On Mon, Jul 23, 2018 at 10:36 AM Susanne Menden-Deuer <smenden at uri.edu>
>> wrote:
>>
>>> Hi all
>>>
>>> I have lost track of who all is interested and participating in the
>>> particle size analysis for EXPORTS, so I thought to send it to all PIs
>>> and we can cull from there.
>>>
>>> If you are interested in an inter-instrument particle size/abundance
>>> verification, read on!
>>>
>>> We had discussed doing an inter-instrument verification during
>>> mobilization next month for both the benchtop instruments (FlowCAM,
>>> IFCB, Flowcytometers, Coulter) as well as the in situ instruments (LISST
>>> etc) and I wanted to bring some cultures along.
>>>
>>> After we had a request for a workshop to OCB denied (lead Lee
>>> Karp-Boss), my lab did a comparison using the instruments we had on hand
>>> (FlowCam, BD Influx, microscope, Coulter Counters model 3 and 4). I
>>> relate some results below. In short, the bench top instruments agree
>>> very well. As far as EXPORTS is concerned, the Guava and IFCB which will
>>> be on board were not included, neither were any of the deployable
>>> instruments.
>>>
>>> Bringing along enough phytoplankton culture volume in a physiologically
>>> valuable state to repeat this in Seattle is prohibitive. My suggestion
>>> is that all suppliers of instruments provide verified standard material
>>> (e.g. beads) that we can run on as many instruments as possible. From
>>> Heidi Sosik I learned that beads are not desirable in terms of flow
>>> cytometry and I could bring a modest amount of Dunaniella sp.
>>>
>>> Then, while in transit, I suggest samples are taken from the inflow
>>> (preferably still within Puget Sound so large particles are more
>>> abundant) and those samples run on both the benchtop and deployable
>>> instruments, so we get a comparison of the particle size distribution
>>> and abundance. This will help us interpret differences from the
>>> different instruments at least when we can reliably say they saw the
>>> same water mass. I'd be happy to write up a protocol.
>>>
>>> Let me know what you think
>>>
>>> Best
>>>
>>> smd
>>>
>>>
>>> Some results from our in lab comparison:
>>>
>>> we quantified cell size and abundance of 9 phytoplankton species from
>>> mono-specific laboratory cultures using optical, electrical and image
>>> based bench top instrumentation. We find generally good agreement in
>>> estimates of both particle concentration and particle size among
>>> instruments. Image based approaches (e.g. microscopy, FlowCAM) delivered
>>> lower cell abundance estimates, because image based instruments
>>> distinguish cells from non-cell particles. Image based approaches also
>>> delivered 10-20% greater estimates of equivalent spherical diameters,
>>> because measurements were based only on in-focus images of the target
>>> species. Particle counters delivered acceptable estimates with much
>>> lower logistical and technical involvement required. Maximum coefficient
>>> of variation for size and abundance measurements did not exceed 15%.
>>>
>>>
>>> --
>>> Susanne Menden-Deuer, Ph.D.
>>> Professor of Oceanography
>>>
>>> University of Rhode Island
>>> Graduate School of Oceanography
>>> Bay Campus
>>> Office: 230 Coastal Institute
>>> Narragansett, RI  02882, USA
>>>
>>>
>>> skype: mendens
>>> http://mendendeuerlab.com/
>>>
>>> _______________________________________________
>>> Exports_pi mailing list
>>> Exports_pi at espo.nasa.gov
>>> https://espo.nasa.gov/lists/listinfo/exports_pi
>>>
>>
>>
>> --
>> ************************************************
>> Emmanuel Boss, Professor
>> School of Marine Sciences
>> 5706 Aubert Hall, University of Maine
>> Orono, ME 04469-5706
>> Tel: 207-581-4378, Cell: 207-745-3061
>> Fax: 207-581-4388
>> emmanuel.boss at maine.edu
>> http://misclab.umeoce.maine.edu/boss/boss.php
>> skype: emmanuel.boss
>> ************************************************
>>
>
>
> --
> ************************************************
> Emmanuel Boss, Professor
> School of Marine Sciences
> 5706 Aubert Hall, University of Maine
> Orono, ME 04469-5706
> Tel: 207-581-4378, Cell: 207-745-3061
> Fax: 207-581-4388
> emmanuel.boss at maine.edu
> http://misclab.umeoce.maine.edu/boss/boss.php
> skype: emmanuel.boss
> ************************************************
> _______________________________________________
> Exports_pi mailing list
> Exports_pi at espo.nasa.gov
> https://espo.nasa.gov/lists/listinfo/exports_pi
>
>
>

-- 
************************************************
Emmanuel Boss, Professor
School of Marine Sciences
5706 Aubert Hall, University of Maine
Orono, ME 04469-5706
Tel: 207-581-4378, Cell: 207-745-3061
Fax: 207-581-4388
emmanuel.boss at maine.edu
http://misclab.umeoce.maine.edu/boss/boss.php
skype: emmanuel.boss
************************************************
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