The TWiLiTE instrument is a compact, rugged direct detection scanning Doppler lidar designed to measure wind profiles in clear air from 18 km to the surface. TWiLiTE operates autonomously on NASA research aircraft (ER-2, DC-8, WB-57, Global Hawk). Initial engineering flight tests on the NASA ER-2 in 2009 demonstrated autonomous operation of all major systems. TWiLiTE will be reconfigured to fly on the NASA Global Hawk as part of the Hurricane and Severe Storm Sentinel Venture Class Mission.