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This dataset provides extensive calibration and in-flight performance data for two Ultra-High Sensitivity Aerosol Spectrometers (UHSAS) used for particle size distribution and volatility measurements during the NASA Atmospheric Tomography Mission (ATom) airborne campaign. UHSAS-1 was equipped with a compact thermodenuder operating at 300 degrees C and UHSAS-2 was operated without a thermodenuder to determine the number and volume fraction of volatile particles. Laboratory studies utilized aerosols from limonene ozonolysis (limon), atomization of ammonium sulfate (AS), and atomization of 2-diethylhexyl (dioctyl) sebacate (DOS). Data include: UHSAS detection efficiency, sizing calibration, performance at a range of pressures and at a range of thermodenuder temperatures, comparison of UHSAS-2 and condensation particle counter (CPC) particle number concentrations, comparisons of UHSAS-1 and UHSAS-2 for dry particle number concentration, surface area and volume collected onboard of a NASA DC-8 aircraft during August 2016, and dry aerosol size distributions for thermodenuded and non-thermodenuded instrument collected in February 2017.